GSS provide the homogenizer for E-coli, the ultra high pressure with very special type of homogenizer valve to give the best in industry result. For E-coli application you can trust on our though knowledge and experience.

Different methods can be used to prepare cell lysates from Escherichia coli (E. coli) cells, for example, including sonication, homogenization, enzymatic lysis and freezing/grinding.

Homogenizers, however, are the most commonly used devices to lyse large quantities of bacteria. A cell suspension is pumped through the minute orifice of a special design homogenizing valve at high pressure, this is followed by the sudden release of the pressure on the suspension, resulting in an instant expansion that provides the turbulence and liquid shear — combined with the high pressure — that breaks the cells.

Compared with other methods, homogenization is both convenient and efficient, offering higher percentage cell disruption rates. GSS homogenizers are able to work continuously, enabling large quantities of cells to be processed at high operational pressures, which eliminates the need to add deoxyribonuclease (DNase) to the cell suspension.

Suggested pressures for typical applications are 800–1000 bar with a single stage homogenizer; multiple passes (2–3) are generally required to achieve adequate lysis. It should be noted that high processing pressures result in a rise in operating temperatures. Cells should, therefore, be cooled (4 °C) prior to use to avoid inactivating proteins by foaming.

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